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Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE)

http://repository.uhamka.ac.id/822/

Herlina B. Setijanti, Herlina

Reconstructed Human Epidermis (RHE) is an artificial epidermis
made in such a way that it resembles human skin, and can be used for the
identification of irritant chemicals, especially for cosmetic and topical medicinal
products. Currently the new RHE is produced by European and American
countries, whose skin physiology is very different from Indonesia. Based on
this, the Center for Research on Drugs and Food, NADFC of Republic of
Indonesia took the initiative to develop the reconstruction of keratinocyte,
melanocytes and fibroblasts cells into RHE adapted to the anatomical and
physiological functions of the skin of Indonesians. RHE is made from an epidermal
layer composed of keratinocyte and melanocytes cells that are reconstructed
with a dermis layer composed of fibroblast and collagen cells.
Keratinocyte, melanocytes and fibroblasts cells are cultured on suitable mediums
by adding a suitable growth medium. To find out that RHE has been
successfully reconstructed, measured percentage of cell life, made histology
preparation to see the existence of cell nucleus, and conducted Immunohistochemical
examination to see existence of integration (bond) between antigen.
From the research results can be seen that keratinocyte cells grown on culture
medium Keratinocyte SFM (IX) with rEGF supplements; melanocyte cells
grown on Melanocyte 254 culture medium with HMGS supplementation; and
fibroblast cells grown on Fibroblast M 106 culture medium with LSGS supplementation.
The percentage of epidermal cell life grew well in the planting of
10 � 104 cells /mL keratinocytes and 0.25 � 104 cells /mL of melanocyte cells
and survived until the 11th day with live cell percentage of 93.45%. In making
preparation for histology with HE staining, there is a cell life in RHE tissue.
Used Immunohistochemical (IHC) examination using cytokeratin 10 antibody
marker to view physiological function of epidermal tissue.

http://repository.uhamka.ac.id/822/1/2019_Chapter_.pdf

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Uji Larvasida Ekstrak n-heksan Daun Zodia (Evodia suaveolens Scheff.) Terhadap Dua Vektor Larva Nyamuk Aedes albopictus dan Culex spp

http://repository.uhamka.ac.id/823/

Ema, Dewanti

Insektisida nabati dapat digunakan untuk mengendalikan dan mencegah penyakit yang disebabkan oleh nyamuk. Salah satu alternatif bahan insektisida
nabati adalah daun zodia (Evodia suaveolens Scheff.) yang telah dikenal sebagai tanaman pengusir nyamuk. Penelitian ini bertujuan untuk mengetahui
efek larvasida dan nilai LC50 dari ekstrak daun zodia terhadap larva nyamuk Aedes albopictus dan Culex spp. Penelitian ini menggunakan ekstrak nheksan
daun zodia dengan variasi konsentrasi 10 ppm; 31,62 ppm; 99,98 ppm; 316,13 ppm; 1000 ppm dan kontrol. Metode yang digunakan adalah
metode bioassay yang telah distandarisasi oleh WHO (2005). Data hasil penelitian dianalisis menggunakan uji probit. Hasil penelitian menunjukkan
bahwa kematian 50% larva nyamuk Aedes albopictus dan Culex spp. (LC50-24 jam) adalah 106,036 ppm dan 116,9110 ppm. Berdasarkan hasil uji probit
nilai LC50 masuk ke dalam rentang 100-149 ppm maka dapat disimpulkan bahwa ekstrak n-heksan daun zodia termasuk ke dalam golongan larvasida
dengan tingkat toksisitas moderat tinggi.

http://repository.uhamka.ac.id/823/1/817-1514-1-SM-halaman-376-381.pdf

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The effects of intensity and duration of aerobic exercise on spatial memory function in male Wistar rats

http://repository.uhamka.ac.id/824/

Neng Tine Kartinah, Tine

BACKGROUND Memory is a vital function of the brain. Aerobic exercise has a positive
effect on memory’s function, but the appropriate combination of intensity and duration
of aerobic exercise is still unknown. This study was aimed to investigate the effect of
optimum combinations of intensity and duration of aerobic exercise on spatial memory
function.

http://repository.uhamka.ac.id/824/1/1825-Article%20Text-24016-3-10-20191004.pdf

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EXOGENT FACTORS AT PT. METRO TARUNA JAKARTA THAT AFFECT EMPLOYEE PERFORMANCE

This study aims to determine the effect of training and work discipline as exogenous factors on employee
performance at PT Metro Taruna Jakarta. The 82 respondents as a sample and data analysis and process
techniques used are validity, reliability, descriptive analysis, normality test, multicollinearity test,
heteroscedasticity test, autocorrelation test, multiple linear regression analysis, simultaneous test,
coefficient test, and correlation coefficient of determination. Based on the results of data processing
Correlation and Determination Coefficients, the nature of the relationship between training, work
discipline and employee performance is affected, because the correlation coefficient is 0.910 and the
coefficient of determination r2 = 0.824 or 82.4%, meaning 82.4% variation or change in employee
performance variables is the contribution of the influence of training and work discipline variables, while
the remaining 17.6% is contributed by the influence of other factors such as compensation, workloads that
are not comparable to payments, lack of motivation, high levels of work stress, low levels of promotion and
others.

http://repository.uhamka.ac.id/825/1/PUBLISHED%20JURNAL%20AGREGAT%20%20comprees.pdf

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Determinants of e-cigarette use among a sample of high school students in Jakarta, Indonesia

http://repository.uhamka.ac.id/826/

Bigwanto, Mouhamad

Objectives
The aim of this study was to determine the predisposing, enabling and reinforcing factors associated with electronic cigarette (ecigarette) use among high school students in Jakarta, Indonesia.
Methods
This cross-sectional study took place in eight high schools in Jakarta, Indonesia. A total of 767 students were recruited by multistage
cluster random sampling. Bivariate and multivariate statistical analyses were employed to determine the associations between sociodemographic, predisposing, reinforcing and enabling factors and e-cigarette use.
Results
Respondents were 54.1% male and the mean age was 16 years old [standard deviation (SD): 1.02]. In this sample, 32.2% of students (n
= 247) had ever used e-cigarettes and 11.8% of students were e-cigarette users (n = 90). Several measured factors were positively
associated with e-cigarette use, including: current smoking of conventional cigarettes [odds ratio (OR): 2.06]; perception that e-cigarettes
are less addictive than conventional cigarettes (OR: 1.98); perception that e-cigarettes do not cause cancer (OR: 2.38); parental
acceptance of e-cigarette use (OR: 3.80); and having enough money to buy e-cigarettes (OR: 3.24). The only variable found that was
negatively associated with e-cigarette use was teacher’s use of e-cigarettes (OR: 0.34).
Conclusions
This study found that student social influences, perceptions about and accessibility to e-cigarettes were significantly positively
associated with e-cigarette use among high school students in Jakarta, Indonesia. This study highlights the importance of educating
students, their parents and teachers regarding safety and potential health hazards of using e-cigarettes. Efforts to implement and
enforce youth access restrictions on e-cigarettes in Indonesia are crucial to preventing further uptake of these products.

http://repository.uhamka.ac.id/826/1/Determinants%20of%20e-cigarette%20use%20among%20a%20sample%20of%20high%20school%20students%20in%20Jakarta%2C%20Indonesia%20_%20International%20Journal%20of%20Adolescent%20Medicine%20and%20Health.pdf

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Biotechnology in Veterinary Medicine

Yusuf, Yusnidar

In various areas of medicine biotechnology is consider as already established approach, but to revolutionize veterinary
practice with the potential in veterinary medicine field it has only begun to emerge. For animal breeding and veterinary medicine
it has proposed new dimensions with the continuous growth of modern biotechnology. To discard any possible genetic disorder i t
ultimately permits to consequently and detect through genome analysis of important breeding species. It can also detect more
reliably and easily the infectious diseases. With improved productivity and health it opens the possibility to generate animals with
the production of transgenic livestock and it introduced a less time taking program of breeding.

http://repository.uhamka.ac.id/827/1/Biotechnology%20in%20Veterinary%20Medicine.pdf

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THE INFLUENCE OF ACADEMIC CULTURE AND TASK COMMITMENT TOWARD LECTURERS’ INNOVATIVENESS

Connie Chairunnisa, Connie

This study aims to investigate the academic culture and task commitment toward lecturers’ innovativeness.
Quantitative survey methods were adopted. To collecting the data, a questionnaire and path analysis were used. Two
hundred fifteen lecturers at several universities in Jakarta (Indonesia) participated in the study. The results indicate
that many academic cultures positively give direct influences to lecturers’ innovativeness, and task commitment
positively gives direct influences to lecturers’ innovativeness. The study concludes that training and mastery of the
use of media are needed to improve the competence and innovation of lecturers in conducting scientific research.

http://repository.uhamka.ac.id/828/1/PUBLISH%20JOURNAL%20DI%20ARPG.pdf

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Construction of recombinant sox2-encoding plasmids that regulate pluripotency of breast cancer stem cells from indonesian patient

http://repository.uhamka.ac.id/812/

Hanifah, Rahmi

A therapy development with recombinant protein is a new and potential innovation to destroy
cancer stem cells (CSCs). Various ways of killing CSCs include the provision of polyvalent antiprotein
antibodies that code pluripotency. Therefore, it takes a mixture of Oct-4, c-Myc, Sox2, and
Klf4 protein antigens that can stimulate the formation of polyvalent antibodies. This study aimed to
construct Sox2 recombinant by identifying the target genes by reverse transcriptase PCR and then
arranging their designs to be inserted into the cloning vector pET101/D-TOPO®. The target gene was
developed by finding the complete sequences of Sox2 nucleotides on the NCBI GenBank. The growth
on LB-ampicillin agar plates was amplified by PCR to obtain colonies with pET101/D-TOPO®
vectors and inserts of the pluripotent gene of CSCs, then the PCR results were observed through
electrophoresis. A total of fifteen colonies have DNA bands with a base pair of about 300 bp in length.
The recombinant clones produced Sox2 genes with a base length of 330 bp.

http://repository.uhamka.ac.id/812/1/11919-33934-1-PB.pdf

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PENGEMBANGAN MODEL PENDIDIKAN KARAKTER DI SEKOLAH MENENGAH PERTAMA

http://repository.uhamka.ac.id/829/

Connie Chairunnisa, Connie

Pendidikan karakter dapat dimaknai sebagai pendidikan nilai, pendidikan budi
pekerti, pendidikan moral, pendidikan watak yang bertujuan mengembangkan
kemampuan peserta didik untuk memberikan keputusan baik-buruk, memelihara
yang baik, dan mewujudkan kebaikan itu dalam kehidupan sehari-hari dengan
sepenuh jiwa dan raga. Selama ini pendidikan karakter di sekolah terintergrasi
pada bidang studi agama, dan bidang studi Pendidikan Kewarganegaraan (PPkn),
belumadapegangankhususbagigurudalammengajarkanpendidikankarakter,
dan belum ada model yang pas agar peserta didik dapat meningkatkan kecerdasan
emosinya. Kecerdasan emosi merupakan bekal penting dalam mempersiapkan
anak menyosong masa depan, sehingga diharapkan siswa lebih mudah dan
berhasil mengahadapi segala macam tantangan kehidupan, termasuk tantangan
untukberhasil secara akademis.Buku ini mengupas tentang 9 indikator pendidikan karakteryangberasaldarinilai-nilailuhuruniversal(CintaTuhan,Kemandirian
dan Tanggung Jawab, Kejujuran, Hormat dan Santun, Dermawan, Percaya Diri
dan Pekerja Keras, Kepemimpinan dan Keadilan, Baik dan rendah hati, Toleransi,
KedamaiandanKesatuan).Kesembilanpilarkarakter tersebut,diajarkansecara
sistematis dalam model pendidikan holistik menggunakan metode knowingthe
good, feeling the good, dan acting the good.

http://repository.uhamka.ac.id/829/1/Pengembangan%20Model%20Pendidikan%20Karakter%20di%20sekolah%20Menengah%20Pertama.pdf

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Pembuatan Obat Kumur Alami Daun Sirih Bagi Anggota Aisyiyah di PRA Cabang Perumnas I dan Jakasampurna

http://repository.uhamka.ac.id/813/

Hanifah, Rahmi

Daun sirih merupakan tanaman obat tradisional yang erat kaitannya dengan kesehatan gigi dan mulut. Daun
sirih diketahui sebagai salah satu Bahan alami yang memiliki kemampuan sebagai obat kumur karena
aktivitasnya sebagai antibakteri penyebab plak gigi. Meskipun telah banyak diketahui khasiatnya,
pemanfaatan dan pembuatan daun sirih sebagai obat kumur bagi masyarakat perlu disosialisasikan untuk
menjaga kesehatan mulut. Pengabdian masyarakat ini dilaksanakan dengan tujuan untuk meningkatkan
pengetahuan mengenai pencegahan bau mulut dengan obat kumur alami dan cara pembuatannya dengan
sederhana kepada masyarakat sehingga dapat meningkatkan derajat kesehatannya secara mandiri serta
meningkatkan pendapatan ekonomi. Mitra yang dipilih menjadi lokasi pengabdian masyarakat didasarkan
pada pertimbangan berdasarkan kepatutan sumber data dan informasi yang dikumpulkan melalui kunjungan
serta diskusi. Target yang dicapai adalah ibu-ibu rumah tangga yang tergolong masyarakat ekonomi
menengah ke bawah. Profil masyarakat di sebagian warga ini tepat untuk mendapatkan tambahan
pengetahuan dan keterampilan yang berguna untuk peningkatan kualitas hidup. Pengabdian masyarakat ini
dibagi menjadi 2 hari, hari pertama berupa pemaparan materi dan hari kedua berupa pelatihan pembuatan
obat kumur. Hasil yang diperoleh yaitu formula obat kumur yang sesuai dengan evaluasi rasa, warna, dan bau
dari orientasi formula. Formula yang terpilih yaitu menggunakan daun sirih dan daun mint (1:1). Dari
pelatihan ini diharapkan anggota Aisyiyah Cabang Perumnas I sebagai stake holder dapat menyebarkan
informasi resep pembuatan obat kumur yang sederhana dan harga terjangkau kepada anggota masyarakat
lainnya.

http://repository.uhamka.ac.id/813/1/3102-217-6980-1-10-20190514.pdf

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